當(dāng)前位置：首頁(yè) > 技術(shù)文章 > Selenotest ELISA kit說(shuō)明書

Selenotest ELISA kit說(shuō)明書

更新時(shí)間：2016-10-25 點(diǎn)擊次數(shù)：2952

immunochemical inligence GmbH?是現(xiàn)在*生產(chǎn)selenoprotein P?試劑盒的廠家，該試劑盒是使用ELISA方法來(lái)測(cè)定人血漿中selenoprotein P蛋白含量，方便檢測(cè)

selenoprotein P (SEPP1)研究背景

亞細(xì)胞定位: Secreted.

組織特異性: Made in the liver and heart and secreted into the plasma. It is also found in the kidney.

翻譯后修飾: Phosphorylation sites are present in the extracellular medium.

相似的序列: Belongs to the selenoprotein P family.

品牌	immunochemical inligence GmbH
產(chǎn)品貨號(hào)	STE
產(chǎn)品名稱	Selenotest ELISA kit
用途	檢測(cè)人類血漿selenoprotein P (SEPP1)蛋白含量
包裝	96孔板
檢測(cè)方法	比色法 Colorimetric (450 nm)
樣品需要量	5 – 10 µL
采樣類型	血漿
檢測(cè)時(shí)間	5 – 6小時(shí)
靈敏度	‹ 8 µg/L (about 500-fold below the serum level of healthy individuals)
檢測(cè)范圍	Approx. 5 – 800 µg/L
定量范圍	Approx. 10 – 400 µg/L (corresponds to 0.2 – 8 mg/L in undiluted sample)
試劑盒保存溫度	Refrigerated (2 – 8度)
保質(zhì)期	6個(gè)月到中國(guó)客戶手上至少3個(gè)月
試劑盒尺寸(L×W×H)	200 × 150 × 95毫米
試劑盒重量	400g
生產(chǎn)地址	德國(guó)
價(jià)格	750歐元，7500RMB
到中國(guó)運(yùn)輸費(fèi)用	2000元
到中國(guó)的時(shí)間	4周左右
代理商	上海起發(fā)實(shí)驗(yàn)試劑有限公司
關(guān)鍵詞	SELENOP selenoprotein P SEPP1
成分組成：
包被好的96孔板	1：Pre-coated, pre-blocked 96 well microplate (1 plate, ready-for-use)
凍干血漿標(biāo)準(zhǔn)品 8管	2：Serum Calibrators, lyophilized (8 tubes, 0.5 mL each)
陽(yáng)性對(duì)照 3管	3：Serum Controls, lyophilized (3 tubes, 0.5 mL each)
陰性對(duì)照 1管	4：Assay Blank, lyophilized (1 tube, 0.5 mL)
重建緩沖液 8ML 一瓶	5：Reconstitution Buffer (1 bottle, 8 mL)
樣品稀釋液20ml一瓶	6：Sample Dilution Buffer (1 bottle, 20 mL)
生物素標(biāo)記抗體125ul	7:Concentrated biotin-conjugated Detection Antibody (DAB), liquid (1 tube, 0.125 mL)
鏈霉親和素過(guò)氧化物酶復(fù)合物	8:Concentrated Streptavidin-Peroxidase Complex (POD), liquid (1 tube, 0.125 mL)
DAB稀釋液11ML	9:DAB Dilution Buffer (1 bottle, 11 mL)
POD稀釋液11ml	10:POD Dilution Buffer (1 bottle, 11 mL)
20x洗滌緩沖液25ml	11:Washing Buffer, 20× concentrate (1 bottle, 25 mL)
POD底物溶液11ml	12:POD Substrate Solution (1 bottle, 11 mL)
終止液11ml	13:Stop Solution (1 bottle, 11 mL)
封板膜4張	14:Plate Cover Foil (4 pieces)
操作指南一份英文	15:Instructions For Use
產(chǎn)品清單一份	16:Product Specification Sheet
質(zhì)量檢查報(bào)告一份	17:Quality Control Certificate

需要額外準(zhǔn)備的產(chǎn)品
移液器及配套吸頭	Calibrated, adjustable precision single- and multichannel pipettes with disposable plastic tips
各種樣品管或者微孔板	Polypropylene 96 well microplate or tubes for diluting samples
混勻器	Vortex mixer
離心機(jī)	Centrifuge for microtubes
洗板機(jī)	Automated microplate washer (option)
搖床	Microplate shaker
分光光度計(jì)	Microplate reader capable measuring absorbance at 450 nm
吸水紙	Absorbent paper towels
純凈水	Laboratory water (type 1 or 2)
相關(guān)軟件	Data analysis and graphing software

References（引用文獻(xiàn)）

[1] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Perspectives in Science. 2015 Feb;3(1-4):23-4. http://dx.doi.org/10.1016/j.pisc.2014.11.013

[2] Ballihaut G, Kilpatrick LE, Kilpatrick EL, Davis WC. Multiple forms of selenoprotein P in a candidate human plasma standard reference material. Metallomics. 2012 Jun;4(6):533-8. http://dx.doi.org/10.1039/c2mt20059g

[3] Ballihaut G, Kilpatrick LE, Davis WC. Detection, identification, and quantification of selenoproteins in a candidate human plasma standard reference material. Anal Chem. 2011 Nov;83(22):8667-74. http://dx.doi.org/10.1021/ac2021147

資料下載：

Poster Presentations

The Selenotest ELISA was first presented at the GMS Jahrestagung 2013 (Gesellschaft für Mineralstoffe und Spurenelemente) and at the 10th International Symposium on Selenium in Biology and Medicine 2013 in Berlin, Germany:

[pdf, 199KB] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Poster Presentation at 10^thInternational Symposium on Selenium in Biology and Medicine 2013, Berlin.

Instructions For Use

[pdf] Arbeitsanleitung (Version 3, DE) [pdf] Instructions for use (Version 3, EN)

Product Specification Sheets

[pdf] Lot:MSP013001 [pdf] Lot:MSP013002 [pdf] Lot:MSP014001 [pdf] Lot:MSP015001

Quality Control (QC) Certificates